The Carbohydrate Components of Gastric Mucopolysaccharides

Chromatographic Studies of the Carbohydrate Components of Gastric and Salivary Mucopolysaccharides.

Linkage of carbohydrate to hydroxyamino acids in mucopolysaccharides and mucoproteins.

1. Glycosidic linkage of carbohydrate to the primary hydroxyl groups of threonine and serine has been established in human blood-group A and Le(a) substances, bovine submaxillary-gland mucin and human pseudomyxomatous mucin. 2. Treatment of these substances in 0.09n-lithium hydroxide at 100 degrees for 1hr. led to beta-elimination at these glycosidic linkages with the resultant formation of alp...

Carbohydrate components of influenza C virions.

The carbohydrate components of influenza C virions grown in chicken kidney (CK) cells were analyzed by gel filtration following exhaustive digestion with Pronase. The [(3)H]glucosamine-labeled glycopeptides were larger and more heterogeneous than those of influenza A/WSN virions; three major size classes (G(1), G(2), and G(3)) were resolved. Treatment with Vibrio cholerae neuraminidase caused a...

Carbohydrate components of lipovitellin of the sand crab Emerita asiatica.

Lipovitellin II (Lv II), the major yolk protein of the anomuran crab Emerita asiatica, was purified using heparin-sepharose affinity column chromatography. The purified Lv II was a glycoprotein as it was stainable with periodic acid-Schiff's reagent. Quantitative analysis of sugars showed the presence of fucose, mannose, galactosamine, N-linked oligosaccharides, as well as O-linked oligosacchar...

The mucopolysaccharides of bovine cornea.

The mucopolysaccharides isolated from various mesodermal tissues have, in several instances, been shown to consist of complex mixtures, and not of single compounds as was believed formerly. In view of the rather contradictory findings reported in the literature, it was thought advisable to reinvestigate the mucopolysaccharide fractions of the cornea. In this paper, the separation of three disti...